{"product_id":"abcam-ab259183","title":"Abcam, ab259183, Human TM9SF4 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTM9SF4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TM9SF4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTM9SF4 also known as Transmembrane 9 Superfamily Member 4 is a multi-pass membrane protein with an approximate molecular mass of 70 kDa. It is localized in the Golgi apparatus and endosomes showing a notable expression in immune-related cells such as macrophages and dendritic cells. TM9SF4 is involved in the transport and sorting of proteins within cellular compartments playing an important role in maintaining intracellular pH homeostasis by modulating ion exchange across membranes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAs part of a larger protein family TM9SF4 is involved in the regulation of cellular processes like endocytosis and exocytosis. It functions as a regulator of phagocytosis a process critical for immune response and cell clearance. Through its action in cellular compartments it contributes to antigen presentation thereby influencing cellular immune responses. Its involvement in these pathways highlights its significance in both normal cellular functions and immune system regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nTM9SF4 influences processes associated with the endocytic pathway and vesicular trafficking. It interacts within cellular networks involving SNARE proteins responsible for vesicle fusion highlighting its role in transport pathways. TM9SF4 also associates with V-ATPase complexes affecting acidic environments necessary for hydrolytic enzymes in endosomes. Through these connections TM9SF4 helps sustain cellular dynamics and integrity.\u003cbr\u003e\nTM9SF4 links to immune-related conditions particularly chronic inflammatory diseases. It affects macrophage function and inflammation making it relevant in disorders such as rheumatoid arthritis. Additionally TM9SF4 alterations associate with tumor microenvironments influencing cancer progression. Through its modulation of phagocytic functions abnormalities in TM9SF4 activity can alter interactions with proteins like TNF-alpha which are important in inflammation and cancer-related pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855784267945,"sku":"ab259183","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259183","provider":"Iright","version":"1.0","type":"link"}