{"product_id":"abcam-ab259188","title":"Abcam, ab259188, Human TMEM160 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTMEM160 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 25 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 25 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TMEM160, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTMEM160 also known as transmembrane protein 160 has a mass of approximately 22 kDa. It primarily functions as a transmembrane protein embedded in the cell membrane. TMEM160 shows expression in various tissues particularly in adipose tissue and to some extent in other cell types. Its specific mechanical function is currently not fully defined but it likely involves interactions with lipid components in the cellular membranes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTransmembrane protein 160 might play a role in energy homeostasis and metabolic processes. Though not part of a larger complex its expression patterns suggest possible involvement in pathways regulating energy storage and release. Further studies are necessary to identify the specific physiological processes and interactions in which TMEM160 participates.\u003cbr\u003e\nPathways\u003cbr\u003e\nTMEM160 links to processes related to energy metabolism and cellular signaling. It interacts with pathways such as the insulin receptor signaling pathway. Research indicates its relationship with proteins like insulin receptor substrate (IRS) proteins which are central to glucose uptake and utilization processes.\u003cbr\u003e\nTMEM160 has been implicated in metabolic disorders particularly obesity and type 2 diabetes. The protein’s expression and function suggest a role in these conditions possibly through its interaction with insulin-like proteins and glucose transporters which are critical in managing blood glucose levels and energy storage. Further exploration into its role could provide insights into therapeutic targets for these metabolic diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845220061353,"sku":"ab259188","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259188","provider":"Iright","version":"1.0","type":"link"}