{"product_id":"abcam-ab259199","title":"Abcam, ab259199, Human TRABD (TraB domain containing) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTRABD KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon4 and 4 bp deletion in exon4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon4 and 4 bp deletion in exon4.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TRABD, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe TraB domain containing protein functions mechanically as a facilitator for DNA transfer during bacterial conjugation. This protein aids in the transport of DNA across the cell membrane playing a critical role in gene exchange processes. Often referred to by various names such as conjugation protein TraB or transfer binding domain protein it has an estimated mass around 50 kDa. TraB is typically expressed in bacteria where it ensures the proper transfer of genetic material between cells harnessing energy from ATP to drive this process efficiently.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTraB domain containing proteins enable horizontal gene transfer which significantly impacts genetic diversity and adaptation in bacterial populations. It constitutes a part of the larger conjugative transfer machinery complex that involves multiple proteins. This complex facilitates the binding and unwinding of DNA which is essential for conjugation. TraB works in association with membrane-associated proteins to ensure successful DNA passage into the recipient cell reflecting its importance in cellular adaptability and survival under various environmental conditions.\u003cbr\u003e\nPathways\u003cbr\u003e\nThese proteins serve a vital function within the bacterial conjugation pathway — a fundamental mechanism contributing to antibiotic resistance spread. TraB operates in coordination with proteins such as TraC or TraG which form part of the transfer complex. These proteins orchestrate the conjugation process ensuring the precise transfer of DNA molecules working as a liaison among the numerous proteins involved in this method. The integration of TraB within these pathways demonstrates its role in promoting gene transfer which bacteria use to acquire new traits rapidly including antibiotic resistance.\u003cbr\u003e\nTraB-related processes have considerable implications on the progression of antibiotic resistance in bacterial infections. The spread of antibiotic-resistant genes through TraB-mediated conjugation poses significant challenges in treating diseases like MRSA (Methicillin-resistant Staphylococcus aureus). TraB connects with other proteins like TraA or TraD facilitating resistance gene dissemination within bacterial communities. Understanding TraB's function and its interactions with these proteins is essential in developing strategies to combat the growing public health threat posed by antibiotic-resistant bacterial strains.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845175070889,"sku":"ab259199","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259199","provider":"Iright","version":"1.0","type":"link"}