{"product_id":"abcam-ab259225","title":"Abcam, ab259225, Human UBE2O knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nUBE2O KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 8 and 2 bp deletion in exon 8.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 8 and 2 bp deletion in exon 8.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-UBE2O, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nUBE2O also known as E2-230K or E2 ubiquitin-conjugating enzyme O is a protein that functions as an E2 ubiquitin-conjugating enzyme. It has a molecular mass of approximately 141 kDa. UBE2O plays a role in transferring ubiquitin to substrate proteins marking them for degradation via the proteasome. This process is important for protein quality control and cellular homeostasis. UBE2O is expressed in various tissues including the brain liver and skeletal muscle indicating its broad physiological significance.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nUBE2O facilitates the regulation of protein turnover by mediating ubiquitination without the need for an E3 ligase. It acts both as an E2 and has E3 activity making it a versatile factor in the ubiquitin-proteasome system. UBE2O also participates in muscle differentiation by targeting specific muscle regulatory factors for ubiquitination. Understanding its function provides insights into the control of muscle mass and function highlighting its contribution to cellular growth and adaptation.\u003cbr\u003e\nPathways\u003cbr\u003e\nUBE2O interacts with essential biological mechanisms notably the ubiquitin-proteasome pathway involved in protein degradation. It also participates in cell cycle regulation by targeting certain cyclin proteins for degradation ensuring proper cell cycle progression. UBE2O works in conjunction with other proteins like ANAPC10 and CDC20 which are important in cell cycle control and proteostasis.\u003cbr\u003e\nUBE2O has associations with conditions such as myopathies and certain cancers. In muscle disorders UBE2O dysregulation can lead to abnormal protein accumulation affecting muscle function. In oncology UBE2O interacts with proteins like P53 which is important in cell cycle regulation and apoptosis. Its role in these disorders underlines its importance as a potential therapeutic target for intervention in these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845189521577,"sku":"ab259225","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259225","provider":"Iright","version":"1.0","type":"link"}