{"product_id":"abcam-ab259244","title":"Abcam, ab259244, Human YIPF3 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nYIPF3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 50 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 50 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-YIPF3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nYIPF3 also known as YIP1 family member 3 is a protein with a molecular mass of approximately 41 kDa. It localizes mainly to the Golgi apparatus and endoplasmic reticulum (ER) within cells. YIPF3 is highly expressed in a variety of tissues including liver kidney and brain indicating its widespread function throughout the body. This protein belongs to the Yip1 family which is associated with membrane trafficking processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe main role of YIPF3 involves regulation of the secretory pathway. It functions as part of a larger protein complex interacting with other members of the YIP family as well as Rab GTPases. YIPF3 contributes to the maintenance and formation of Golgi morphology ensuring proper vesicle trafficking between the ER and Golgi compartments. This activity is essential for efficient processing and transport of proteins and lipids in the cell.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of YIPF3 can prominently be seen in the secretory and transport pathways of eukaryotic cells. It associates with the Rab GTPase pathway influencing vesicle budding and fusion essential for cellular transport mechanisms. YIPF3 works alongside Rab proteins to facilitate correct vesicular movement and signal transduction ensuring proper protein sorting and secretion.\u003cbr\u003e\nDisruptions involving YIPF3 have connections to neurodegenerative diseases and certain cancers. Dysfunction in YIPF3 activity can lead to impaired vesicle trafficking contributing to neuron degeneration seen in neuronal disorders. Additionally alterations in YIPF3 expression levels have been observed in some oncological diseases highlighting its connection to cancer pathways. Its interplay with proteins such as Rab GTPases further illustrates the potential for broader impacts on disease development and progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855785480361,"sku":"ab259244","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab259244","provider":"Iright","version":"1.0","type":"link"}