{"product_id":"abcam-ab261723","title":"Abcam, ab261723, Human CBX4 knockout HEK-293 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nCBX4 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. Knockout achieved by CRISPR\/Cas9; X = 7 bp deletion, 1 bp insertion, 10 bp deletion. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by CRISPR\/Cas9; X = 7 bp deletion, 1 bp insertion, 10 bp deletion\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nWestern blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-CBX4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCBX4 also known as Chromobox protein homolog 4 or Polycomb 2 acts mechanically as part of the Polycomb group (PcG) proteins. It weighs approximately 60 kDa and it is found in nuclear matrix where it participates in chromatin remodeling. CBX4 shows significant expression in tissues like liver brain and lung indicating its wide importance to cellular function. By binding to methylated histones especially H3K27me3 CBX4 influences gene silencing and epigenetic regulation important for maintaining cellular identity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCBX4 operates as a component of the Polycomb Repressive Complex 1 (PRC1) which plays role in controlling gene expression patterns during development. In this complex CBX4 interacts with other core components such as BMI1 and RING1 to facilitate monoubiquitination of histone H2A leading to transcriptional repression. This ability of CBX4 to modulate epigenetic states highlights its importance in critical developmental processes and differentiation.\u003cbr\u003e\nPathways\u003cbr\u003e\nCBX4 significantly influences gene regulatory networks involved in cell cycle and apoptosis pathways. In the cell cycle regulation CBX4 interacts with important proteins such as Cyclin D1 impacting cell proliferation. In apoptosis CBX4 has roles linked to the p53 pathway by modulating transcriptional activity which influences cellular fate. These pathways underline the significance of CBX4 in maintaining balance between cell growth and death.\u003cbr\u003e\nThe deregulation of CBX4 expression has been associated with cancers like hepatocellular carcinoma and breast cancer. CBX4 potentially interacts with proteins such as p21 and p16 whose pathways play significant roles in cancer development. This underlines its role as a potential biomarker or therapeutic target in cancer treatment strategies. Understanding the function and regulation of CBX4 could offer insights in devising novel treatments for these malignancies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845147611305,"sku":"ab261723","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab261723","provider":"Iright","version":"1.0","type":"link"}