{"product_id":"abcam-ab261779","title":"Abcam, ab261779, Human SFN (14-3-3 sigma) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSFN KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SFN, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\n14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine\/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\n14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K\/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.\u003cbr\u003e\nDisruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845080961193,"sku":"ab261779","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab261779","provider":"Iright","version":"1.0","type":"link"}