{"product_id":"abcam-ab261832","title":"Abcam, ab261832, Human BBC3 (PUMA) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nBBC3 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 2 and 1 bp insertion in exon 2 and 22 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 2 and 1 bp insertion in exon 2 and 22 bp deletion in exon 2,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-BBC3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPUMA also known as BBC3 (Bcl-2-binding component-3) is a pro-apoptotic protein that plays important mechanical roles in promoting apoptosis. It has a mass of approximately 23 kDa. PUMA is expressed widely in various tissues including the lung liver and heart. The protein works mechanically by interacting with anti-apoptotic Bcl-2 family members releasing pro-apoptotic factors like Bax and Bak which engage mitochondria to trigger cell death.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPUMA functions as a pivotal mediator of apoptosis in response to diverse stimuli such as DNA damage and oncogenic stress. As part of the Bcl-2 family PUMA does not typically form a complex with other proteins but functions directly by binding and neutralizing anti-apoptotic Bcl-2 family proteins. This action results in the release of caspase-activating factors from mitochondria essential for programmed cell death.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeveral cellular pathways incorporate PUMA to regulate apoptosis. PUMA is notably included in the p53 pathway where p53 transcriptionally activates PUMA following cellular stress or DNA damage. The presence of related proteins like Bax and Bim in the mitochondrial apoptotic pathway links these signals to the mitochondrial death machinery facilitating apoptosis.\u003cbr\u003e\nPUMA plays significant roles in cancer and neurodegenerative conditions. PUMA overexpression induces excessive cell death relevant in the pathology of neurodegenerative diseases. In cancer PUMA's interactions with proteins like p53 highlight its involvement in tumor suppression suggesting that dysregulation of PUMA expression can contribute to tumorigenesis when anti-apoptotic signals predominate potentially leading to chemoresistance.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845082468521,"sku":"ab261832","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab261832","provider":"Iright","version":"1.0","type":"link"}