{"product_id":"abcam-ab262500","title":"Abcam, ab262500, Human PICALM knockout U-2 OS cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nPICALM KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Knockout achieved by CRISPR\/Cas9. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:U-2 OS,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Bone,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by CRISPR\/Cas9,\u003cbr\u003e\nDisease:Osteosarcoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PICALM, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPICALM also known as Phosphatidylinositol binding clathrin assembly protein is a protein weighing approximately 70 kDa. It plays an important role in clathrin-mediated endocytosis where it helps assembly of the clathrin coat on the cytoplasmic face of the cell membrane. Expression of the PICALM gene is found in various tissues with significant levels in the brain lungs and spleen. The gene encoding PICALM is located on chromosome 11.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPICALM is essential in the trafficking of molecules within cells particularly in the endocytosis and endosomal sorting pathways. This protein is part of large protein complexes assembled during the formation of vesicles. Through these complexes PICALM influences the transport and recycling of membrane receptors and other molecules ensuring proper cellular functions. The clathrin coat assembly and disassembly rely heavily on its function for effective cellular trafficking processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nPICALM integrates into the clathrin-mediated endocytosis and vesicle-mediated transport pathways. It works alongside proteins such as adaptin and dynamin to facilitate the internalization of proteins and later processing within cellular compartments. In the context of cellular signaling and homeostasis these pathways involving PICALM contribute significantly to receptor recycling and downregulation maintaining proper cellular responses to external stimuli.\u003cbr\u003e\nPICALM has been linked to Alzheimer's disease and acute myeloid leukemia (AML). In Alzheimer's disease altered PICALM function or expression can affect amyloid precursor protein processing implicating proteins such as presenilins and tau as well. For acute myeloid leukemia the PICALM-MLLT10 fusion protein resulting from chromosomal translocations can contribute to leukemogenesis by disrupting normal gene regulation. These associations highlight the diverse roles and importance of PICALM in both normal physiology and pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845124411561,"sku":"ab262500","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab262500","provider":"Iright","version":"1.0","type":"link"}