{"product_id":"abcam-ab263086","title":"Abcam, ab263086, Human ANKRD13A knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nANKRD13A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 7 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 7 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ANKRD13A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nANKRD13A also known as Ankyrin Repeat Domain 13A is a protein weighing approximately 114 kDa. It contains ankyrin repeat motifs that facilitate protein-protein interactions making it important for cellular processes. ANKRD13A expresses widely across tissues with the highest levels in the brain heart and skeletal muscle. These repeat motifs are characteristic of proteins that participate in a variety of cellular functions suggesting ANKRD13A's role in maintaining cellular architecture and signaling.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nANKRD13A interacts with several proteins implicating it in cellular adhesion and signaling pathways. It forms part of a complex involved in endocytosis and intracellular trafficking. These processes are essential for maintaining cellular communication and signaling balance. ANKRD13A's protein interactions suggest functions in regulating cell membrane dynamics and vesicle transport which are critical for constant cellular adaptation to environmental signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nANKRD13A plays a significant role in endocytosis and neurotransmitter trafficking. It interacts within the endocytic pathway to regulate the internalization and sorting of membrane proteins. ANKRD13A has connections to clathrin-mediated endocytosis and may influence synaptic vesicle recycling. The protein associates with clathrin and adaptor protein complexes indicating its contribution to maintaining neurotransmitter homeostasis in neural pathways.\u003cbr\u003e\nANKRD13A has associations with neurological and cardiovascular conditions. Deregulation or mutations in ANKRD13A may contribute to neurodegenerative diseases such as Alzheimer's and heart diseases. The protein's link to dynamin and other cytoskeletal proteins is critical since these proteins play roles in amyloid precursor protein (APP) processing and endocytic pathways related to these illnesses. Understanding how ANKRD13A interacts in these contexts can help in exploring therapeutic options for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855781122217,"sku":"ab263086","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263086","provider":"Iright","version":"1.0","type":"link"}