{"product_id":"abcam-ab263160","title":"Abcam, ab263160, Human COA4 (CHCHD8) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nCOA4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-COA4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCHCHD8 is a mitochondrial protein also known as Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 8. It features a molecular mass of approximately 23 kDa. Researchers find CHCHD8 expressed predominantly in the mitochondria of skeletal muscle heart and brain tissues. This protein contributes to mitochondrial function and organization assisting in the maintenance of cellular energy and balance.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCHCHD8 acts as a component of the mitochondrial intermembrane space bridging complex (MICOS). This complex plays a pivotal role in maintaining mitochondrial architecture and integrity. CHCHD8 contributes to the folding and assembly of other mitochondrial proteins. Its interactions within the MICOS complex reveal its influence on cristae morphology which is essential for the proper function of the electron transport chain.\u003cbr\u003e\nPathways\u003cbr\u003e\nCHCHD8 takes part in mitochondrial biogenesis and cellular respiration pathways. These pathways are important for cell energy production and metabolic balance. CHCHD8 participates alongside proteins such as CHCHD3 and SAMM50 in regulating the structure and function of mitochondria integrating signals related to energy requirements and reactive oxygen species handling.\u003cbr\u003e\nCHCHD8 has a connection with neuromuscular diseases and mitochondrial myopathies. Mutations or disruptions in CHCHD8 can lead to impaired mitochondrial function contributing to disease pathology. CHCHD8 is linked with CHCHD10 another protein implicated in similar disorders highlighting their combined importance in mitochondrial and neuromuscular health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845214097577,"sku":"ab263160","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263160","provider":"Iright","version":"1.0","type":"link"}