{"product_id":"abcam-ab263215","title":"Abcam, ab263215, Human GEMIN6 (SIP2) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nGEMIN6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2.,\u003cbr\u003e\nDisease:Adenoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GEMIN6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGemin6 also known as SIP2 (Survival of Motor Neurons Interacting Protein 2) is a protein that plays a role in the assembly of small nuclear ribonucleoproteins (snRNPs). It has a molecular weight of approximately 39 kDa and is expressed mainly in the brain and spinal cord. This protein facilitates interactions within the SMN (Survival Motor Neuron) complex which is essential for the biogenesis of snRNPs necessary for pre-mRNA splicing.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGemin6\/SIP2 functions as a component of the SMN complex which is key to snRNP maturation and assembly. The SMN complex plays an important role in the cellular machinery responsible for processing pre-mRNA to mature mRNA. Gemin6\/SIP2 binds specifically to core proteins in snRNPs aiding in their stability and function. It ensures accurate splicing of mRNA which is critical for proper gene expression and cellular function.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeveral molecules are associated with the Gemin6\/SIP2's role in spliceosomal snRNP assembly. It is involved in the pre-mRNA splicing pathway and links to the RNA transport pathway via the SMN complex. Proteins such as Gemin2 and Sm proteins relate closely to Gemin6\/SIP2 through these pathways forming a functional network critical for RNA processing and regulation.\u003cbr\u003e\nGemin6\/SIP2 has connections to neurodegenerative conditions such as spinal muscular atrophy (SMA). SMA is characterized by the loss of motor neurons which Gemin6\/SIP2 influences through its function in the SMN complex. The dysfunction of this complex due to mutations or imbalances can contribute to the symptoms of SMA. Gemin6\/SIP2 connects with SMN protein whose deficiency directly associates with the pathogenesis of SMA highlighting the significance of this protein in neurological health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847383863465,"sku":"ab263215","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263215","provider":"Iright","version":"1.0","type":"link"}