{"product_id":"abcam-ab263227","title":"Abcam, ab263227, Human HERC4 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nHERC4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-HERC4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHERC4 also known as HECT and RLD domain-containing E3 ubiquitin protein ligase 4 is a protein that mechanically functions as an E3 ubiquitin ligase. Weighing approximately 120 kDa HERC4 facilitates the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to substrate proteins tagging them for degradation by the proteasome. The protein is expressed in various tissues including the brain heart and lung indicating its broad functional significance across different biological systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHERC4 plays an important role in regulating protein turnover and cellular processes. As part of the ubiquitin-proteasome system it ensures protein quality control and homeostasis by targeting misfolded or damaged proteins. While HERC4 does not traditionally form complexes its activity influences the stability and function of other proteins within the cell. This implicates HERC4 in not only cellular maintenance but also processes such as cell cycle regulation and signal transduction.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe ubiquitin-proteasome pathway involves HERC4 contributing to the degradation of regulatory proteins that control various cellular activities. The protein interacts with pathways like cell cycle progression and stress response. It maintains a functional relationship with proteins such as p53 a known tumor suppressor and other E3 ligases that manage the proteolytic balance in the cell demonstrating its integration into critical regulatory networks.\u003cbr\u003e\nHERC4 has connections to cancer and neurodegenerative diseases. In cancer aberrant ubiquitination activities involving HERC4 can disrupt normal protein regulation leading to uncontrolled cell growth. It may interact with oncogenic proteins that escape degradation promoting tumor progression. In neurodegenerative disorders such as Parkinson's disease dysregulation of the ubiquitin-proteasome system potentially involving HERC4 connects with proteins like Parkin impacting neuronal survival and function. Understanding HERC4's role in these pathways provides insights into potential therapeutic targets for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845192798377,"sku":"ab263227","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263227","provider":"Iright","version":"1.0","type":"link"}