{"product_id":"abcam-ab263285","title":"Abcam, ab263285, Human NT5C knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nNT5C KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 25 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 25 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NT5C, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNT5C also known as dNT-1 is a cytosolic enzyme with a molecular weight of approximately 41 kDa. It functions by dephosphorylating nucleoside monophosphates into nucleosides and its activity is important for nucleotide metabolism in cells. NT5C is expressed in various tissues including liver brain and skeletal muscle enabling these tissues to efficiently manage nucleotide balance.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNT5C plays a role in maintaining cellular nucleotide pools impacting DNA and RNA synthesis. The enzyme is not part of a larger protein complex and primarily functions independently in nucleotide salvage pathways. Its activity influences the availability of nucleosides which are necessary for cellular replication and repair processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nNT5C contributes to purine and pyrimidine metabolism. It is involved in the nucleotide salvage pathways collaborating with other enzymes like adenosine deaminase and cytidine deaminase. These pathways play essential roles in recycling nucleotides and maintaining the nucleoside balance critical for cellular function.\u003cbr\u003e\nNT5C has implications in cancer and neurological diseases. Abnormal activity of NT5C might contribute to nucleotide imbalances observed in certain cancers. Its role in dephosphorylation in the brain links it with neurodegenerative conditions potentially involving proteins like adenosine kinase. Understanding its function may offer insights into therapeutic strategies for these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855784366249,"sku":"ab263285","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263285","provider":"Iright","version":"1.0","type":"link"}