{"product_id":"abcam-ab263328","title":"Abcam, ab263328, Human RHOG knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRHOG KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RHOG, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRHOG also known as ras homolog gene family member G is a small GTPase with a molecular mass of approximately 21 kDa. It belongs to the Rho family which plays a role in various cellular functions. RHOG is highly expressed in hematopoietic cells with significant presence in macrophages and neutrophils. This protein is also found in various tissues where it assists in cell movement and cytoskeletal rearrangements.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRHOG regulates cell shape and motility. It is involved in forming membrane protrusions such as filopodia and lamellipodia. RHOG can act as a part of a protein complex with other GTPases. It controls actin cytoskeleton dynamics by activating pathways needed for cell migration and chemotaxis. This protein significantly aids immune cell function during immune surveillance and inflammatory responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nRHOG interacts with diverse signaling cascades. It activates the Rac1 pathway influencing cellular migration and growth processes. RHOG collaborates with proteins like PI3-kinase in these pathways contributing to the regulation of cytoskeletal organization. RHOG involvement in these signaling networks ensures cells respond aptly to external cues maintaining cellular integrity and function.\u003cbr\u003e\nRHOG is implicated in cancer and chronic inflammatory diseases. Abnormal RHOG expression or activity can lead to excessive cell movement and invasion often linked to cancer metastasis. Additionally it relates to chronic inflammatory responses where dysregulation may exacerbate disorders such as rheumatoid arthritis. In these conditions RHOG interacts with proteins like RhoA to mediate changes in cell behavior that drive disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845074702505,"sku":"ab263328","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263328","provider":"Iright","version":"1.0","type":"link"}