{"product_id":"abcam-ab263335","title":"Abcam, ab263335, Human RRP36 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRRP36 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 19 bp deletion in exon1 and 1 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 19 bp deletion in exon1 and 1 bp insertion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RRP36, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRRP36 also known as RRP36 homolog is a protein involved in ribosome biogenesis. It has a molecular mass of approximately 33 kDa. This protein is expressed chiefly in the nucleolus of eukaryotic cells. It plays an important mechanical role in the early stages of processing of precursor rRNA. RRP36 is essential for the maturation of the 90S pre-ribosome an important step in ribosome synthesis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe RRP36 protein functions as part of the small subunit (SSU) processome a large ribonucleoprotein complex. This complex plays a significant role in the early cleavage steps of pre-rRNA necessary for forming functional ribosomes. By contributing to pre-rRNA processing RRP36 aids in the efficient production of ribosomal RNA a core component of ribosomes. This precise processing is required for ribosome assembly and cellular protein synthesis.\u003cbr\u003e\nPathways\u003cbr\u003e\nRRP36 integrates into the nucleolar small subunit processome-related pathway influential in ribosome biogenesis. This pathway involves many proteins including UTP4 and UTP15 which closely associate with RRP36 in the SSU processome. The successful operation of this pathway is critical for linking ribosome production to overall cellular growth proliferation and metabolism.\u003cbr\u003e\nRRP36 shows a connection with disorders resulting from defects in ribosome biogenesis. Impaired ribosome production can contribute to ribosomopathies which include diseases such as Diamond-Blackfan anemia. In this context RRP36 operates alongside other ribosomal biogenesis-related proteins such as UTP18 emphasizing its role in normal cellular functioning and the consequences arising from its dysregulation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845182869673,"sku":"ab263335","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263335","provider":"Iright","version":"1.0","type":"link"}