{"product_id":"abcam-ab263343","title":"Abcam, ab263343, Human SERP1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSERP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SERP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Stress-Associated Endoplasmic Reticulum Protein 1 (SERP1) also known as Ribosome-Associated Membrane Protein 4 (RAMP4) performs important mechanical functions within the cell. With a mass of about 24 kDa SERP1 assists in the stabilization of newly synthesized polypeptides ensuring their proper folding and maturation within the endoplasmic reticulum. This protein is ubiquitously expressed in many tissues reflecting its broad role in maintaining cellular homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe function of SERP1 involves the interaction with ribosomes and the endoplasmic reticulum's membrane-associated components. SERP1 helps manage and support the correct folding of nascent proteins especially during cellular stress conditions. It does not form part of a larger protein complex but works closely with other stress response proteins to regulate protein synthesis integrity under adverse conditions.\u003cbr\u003e\nPathways\u003cbr\u003e\nStress response mechanisms and protein synthesis serve as essential biological contexts for SERP1's function. SERP1 is active in the endoplasmic reticulum stress response pathway helping mitigate misfolded protein accumulation during conditions such as unfolded protein response (UPR). It interacts with proteins like BiP\/GRP78 which plays a part in these pathways coordinating a cell's adaptation to stress.\u003cbr\u003e\nThe malfunction of SERP1 impacts neurodegenerative diseases and metabolic disorders. Research links SERP1 to conditions such as Alzheimer's disease where inefficiencies in protein handling may worsen pathology. Moreover disruptions in SERP1 activity can associate with metabolic disorders like diabetes involving proteins such as PERK which participates in managing cellular stress responses similar to SERP1.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847383896233,"sku":"ab263343","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263343","provider":"Iright","version":"1.0","type":"link"}