{"product_id":"abcam-ab263375","title":"Abcam, ab263375, Human SUGP2 (SFRS14) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSUGP2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 459 bp deletion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 459 bp deletion in exon3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SUGP2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSFRS14 also known as SUGP2 is a protein involved in RNA splicing processes. It facilitates the assembly of spliceosomal complexes which are important in removing introns from pre-mRNA transcripts. The molecular mass of SFRS14 is approximately 111 kDa. This protein expresses widely in human tissues with significant expression in the brain heart and reproductive tissues. Its function in these tissues indicates it plays an important role in post-transcriptional gene regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSFRS14 influences the regulation of alternative splicing by interacting with other splicing factors. It acts as part of the spliceosome complex which is responsible for the precise cutting and joining of exons from mRNA precursors. SFRS14 ensures the correct splicing of transcripts related to various cellular functions. Its role in alternative splicing helps generate protein diversity which is essential in maintaining cellular processes and adaptation to different physiological conditions.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis protein takes part in the mRNA processing pathway and the gene expression pathway. In the mRNA processing pathway SFRS14 assists in accurately assembling the spliceosome ensuring proper splicing of precursor mRNA. Relating to other proteins in these pathways SFRS14 interacts with members of the SR protein family which includes proteins like SFRS1 and SFRS2. These interactions highlight its contribution to the regulation of gene expression through mRNA maturation.\u003cbr\u003e\nSeveral cases relate improper function of SFRS14 to specific pathologies like cancer and neurological conditions. In cancer aberrant alternative splicing events which involve SFRS14 alter the expression of genes that control cell growth and apoptosis. The SFRS14 protein connects to the BCL2 family of proteins which play a role in apoptotic processes in cancer cells. Similarly disruptions in SFRS14 function have been observed in neurodegenerative diseases where it may affect neuronal survival and plasticity due to faulty splicing of essential neuronal transcripts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847390056617,"sku":"ab263375","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263375","provider":"Iright","version":"1.0","type":"link"}