{"product_id":"abcam-ab263391","title":"Abcam, ab263391, Human TMEM30A (Transmembrane protein 30A) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTMEM30A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 7 bp deletion in exon1 and Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 7 bp deletion in exon1 and Insertion of the selection cassette in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TMEM30A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTransmembrane protein 30A also known as TMEM30A has an important role in cellular function as a component of the cell membrane. It is a 31 kDa protein that facilitates the transportation of certain lipids from the outer to the inner leaflets of the plasma membrane functioning as a chaperone alongside other transporters. TMEM30A shows expression in various tissues with notable levels in the brain heart and kidney reflecting its diverse cellular roles in different organ systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTMEM30A is critical in maintaining lipid asymmetry in cell membranes. It acts within a larger complex with flippases such as ATP11 and ATP8 proteins enabling the efficient translocation of phospholipids. This activity affects membrane curvature and flexibility which is essential for cellular processes like vesicle formation endocytosis and maintenance of membrane integrity. Disruption in TMEM30A function can result in altered cellular homeostasis impacting cell viability and signaling.\u003cbr\u003e\nPathways\u003cbr\u003e\nTMEM30A plays a significant part in the phospholipid flipping pathway chiefly interacting with ATP11C in energizing the lipid translocation process. This pathway is fundamental for activities like signal transduction and apoptosis where these lipids serve as substrates or signaling molecules. The protein also interrelates with the calcium signaling pathway wherein altered phospholipid distribution can influence calcium ion channels therefore impacting cellular responses and homeostasis.\u003cbr\u003e\nTMEM30A has associations with neurological disorders and cardiovascular diseases. Disturbances in its function link closely with Alzheimer's disease where phosphatidylserine asymmetry is compromised affecting neuronal communication. TMEM30A is also relevant in the context of heart failure interacting with proteins like ATP8A1 where improper lipid transport can impair cardiac function. Dysregulation of these pathways due to TMEM30A mutations highlights its importance in maintaining physiological health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845184475305,"sku":"ab263391","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263391","provider":"Iright","version":"1.0","type":"link"}