{"product_id":"abcam-ab263392","title":"Abcam, ab263392, Human TMEM59 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTMEM59 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Insertion of the selection cassette in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TMEM59, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTMEM59 also known as Transmembrane Protein 59 plays a role in protein glycosylation and modulation of the cell surface expression of certain proteins. It has a molecular mass of approximately 39 kDa. TMEM59 is expressed in a variety of tissues including the brain lung and kidney where it localizes to the endoplasmic reticulum and Golgi apparatus. The protein's structure suggests membrane spanning properties that contribute to its function in cell regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTMEM59 contributes to proper neuronal function by modulating the cell surface expression of proteins involved in neuron-neuron communications. This regulation has implications for membrane protein function and synaptic stability. TMEM59 participates in certain bionetworks interacting with protein complexes that influence cell signaling pathways. Such interactions play significant roles in controlling protein trafficking and localization within cells impacting the cellular response to environmental signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nTMEM59 influences processes like the Wnt signaling pathway affecting cell fate decisions and embryonic development. It interacts with proteins such as β-catenin and affects their stability and function. TMEM59 also participates in the autophagy-dependent pathways modulating cellular mechanisms involved in the degradation of damaged proteins and organelles therefore maintaining cellular homeostasis.\u003cbr\u003e\nTMEM59 has links to neurodegenerative diseases including Alzheimer's disease and schizophrenia. TMEM59's regulation of proteins like APP (Amyloid precursor protein) is significant. Disruptions in its function may contribute to aberrant protein aggregation commonly seen in Alzheimer's disease pathology. Additionally disruptions in TMEM59 expression or function could affect cognitive processes and synaptic connections related to schizophrenia as it influences the trafficking of receptor proteins important for synaptic signaling.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847390154921,"sku":"ab263392","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263392","provider":"Iright","version":"1.0","type":"link"}