{"product_id":"abcam-ab263398","title":"Abcam, ab263398, Human TRAPPC2L knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTRAPPC2L KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TRAPPC2L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTRAPPC2L also known as trafficking protein particle complex 2L is a protein approximately 52 kDa in mass. It plays a role in vesicle-mediated transport processes. TRAPPC2L is widely expressed in various tissues but shows higher expression in the adrenal gland and testis. Its mechanical function involves the regulation of intracellular protein trafficking which is important for transporting proteins to their proper destinations within cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein is a component of the TRAPP complex a multi-subunit protein assembly involved in the tethering of transport vesicles before fusion with their target membranes. TRAPPC2L contributes to the function and structural integrity of this complex which is essential for maintaining cellular organization and exchanging materials between different cellular compartments. This involvement implies that TRAPPC2L plays a significant part in cellular logistics and maintenance of homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nTRAPPC2L engages in the secretory pathway interfacing with the SNARE proteins necessary for vesicle fusion. This protein also links to the Ypt\/Rab GTPases pathway which regulates vesicular transport and budding processes. Through these interactions TRAPPC2L associates with proteins like TRAPPC2 and TRAPPC3 enhancing vesicle tethering and trafficking dynamics.\u003cbr\u003e\nTRAPPC2L shows associations with medical conditions such as congenital disorders of glycosylation and nephrotic syndrome. Deficient or dysfunctional TRAPPC2L can disrupt vesicle transport pathways leading to cellular dysfunction and disease. The pathways connected to TRAPPC2L can also involve other proteins like TRAPPC2 and TRAPPC9 which contribute to the pathology through their interconnected roles in similar transport processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855776403625,"sku":"ab263398","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263398","provider":"Iright","version":"1.0","type":"link"}