{"product_id":"abcam-ab263399","title":"Abcam, ab263399, Human TSSC1 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nEIPR1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon4 and Insertion of the selection cassette in exon4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon4 and Insertion of the selection cassette in exon4.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-EIPR1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTSSC1 also known as Tumor Suppressor Candidate 1 plays a role in cellular machinery by facilitating protein complex formation. This protein has a molecular mass of approximately 47 kDa. Researchers find its expression in a wide range of tissues notably in highly metabolic active tissues. TSSC1's function involves maintaining structural integrity within the cell by aiding the assembly of multi-protein complexes which is essential for proper cellular operations.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTSSC1 contributes to cellular homeostasis and development. It is part of the Commander complex which is important for endosomal sorting and trafficking. By aiding the assembly and stabilization of this complex TSSC1 supports cellular processes that rely on proper endosomal transport. This function maintains cellular activities that involve the movement and sorting of various biomolecules impacting growth and response mechanisms within cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nTSSC1 engages in intracellular transport and signal transduction pathways. It plays a significant role in the endosomal sorting pathway which controls material transport within cells. TSSC1 works closely with proteins like VPS26 and COMMD proteins which are essential players in endosomal sorting complexes. These interactions ensure efficient intracellular communication and materials handling affecting signal transduction pathways that mediate responses to external and internal signals.\u003cbr\u003e\nMutations and dysregulation of TSSC1 link to certain developmental disorders. Aberrant functioning of the endosomal sorting and trafficking process can lead to neurodevelopmental disorders due to its important role in neuronal growth and maintenance. Additionally TSSC1 may relate to cancer where its interaction with tumor suppressor pathways is altered. Disruptions in associated pathways or with proteins like TRIM25 which is involved in cellular stress responses can contribute to cancer development due to loss of regulatory control over cell proliferation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845184737449,"sku":"ab263399","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263399","provider":"Iright","version":"1.0","type":"link"}