{"product_id":"abcam-ab263401","title":"Abcam, ab263401, Human TTLL12 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTTLL12 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TTLL12, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTTLL12 also known as Tubulin Tyrosine Ligase Like 12 is an enzyme that belongs to the tubulin tyrosine ligase-like (TTLL) family. This protein functions mechanically by catalyzing the polyglutamylation of target proteins especially tubulin impacting microtubule stability and interaction. TTLL12 has a mass of approximately 60 kDa and is predominantly expressed in the brain and testis although its expression is noted in other tissues as well. The involvement of TTLL12 in these tissues suggests its significance in specialized cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTTLL12 influences neuronal and cellular functions through its capacity to alter the microtubule network. It participates in the polyglutamylation activity that regulates microtubule dynamics synaptic stability and intracellular transport playing critical roles in neurodevelopment and cellular organization. Although TTLL12 itself does not commonly form large complexes its enzymatic activity impacts other microtubule-modifying complexes indirectly affecting cellular architecture and signaling pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nTTLL12 participates notably in the microtubule-network pathway and the regulation of cytoskeleton organization. Its activity modulates the polymerization and depolymerization dynamics of microtubules impacting cell cycle and neuronal signaling. TTLL12's function is related to other tubulin-modifying enzymes such as TTLL1 and TTLL5 which share a role in tubulin modification thereby affecting cellular processes like mitosis and intracellular trafficking.\u003cbr\u003e\nTTLL12's aberrant activity or expression has connections to neurodegenerative diseases and male infertility. For example its dysfunction in the brain is associated with tauopathies where improper microtubule regulation contributes to disease pathology. Additionally TTLL12's function relates to proteins like tau and other microtubule-associated proteins (MAPs) which play roles in maintaining neuronal structure and function. Understanding its connections to these conditions may shed light on potential therapeutic strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855777091753,"sku":"ab263401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263401","provider":"Iright","version":"1.0","type":"link"}