{"product_id":"abcam-ab263421","title":"Abcam, ab263421, Human WIBG (PYM) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPYM1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 19 bp deletion in exon1 and 1 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 19 bp deletion in exon1 and 1 bp insertion in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PYM1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPYM also known as PYM1 or Partner of Y14 and Magoh is a mechanistic component involved in the regulation of nonsense-mediated mRNA decay (NMD). It has a molecular mass of approximately 29 kDa. PYM is mainly expressed in the cytoplasm of cells particularly in regions with high cellular turnover such as the brain and testis. PYM interacts with the exon junction complex adjusting mRNA metabolism by binding to key components Y14 and Magoh facilitating mRNA surveillance.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe PYM protein has significant influence on the mRNA surveillance machinery working as a modulator of NMD pathways. PYM is closely linked with the exon junction complex functioning with Y14 and Magoh to ensure mRNA molecules are properly processed for translation. It does not work alone; it forms part of a specialized complex that recognizes faulty mRNA with premature stop codons ensuring the upkeep of cellular mRNA quality.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe PYM protein functions within the mRNA surveillance pathway which preserves integrity of gene expression. PYM interacts with Upf1 which plays a major role in detecting and initiating the degradation of faulty mRNA. It also connects with components of the mRNA export pathway ensuring only properly spliced and processed transcripts leave the nucleus. The partnership between PYM and other exon junction complex proteins contributes significantly to maintaining efficient and accurate gene expression protocols.\u003cbr\u003e\nErrors in PYM function associate with neurological disorders due to its expression in the brain. Mutations or dysregulation here can affect mRNA quality control leading to ailments such as intellectual disability. PYM's interactions with Upf1 link it to disorders where NMD pathway errors occur further highlighting its importance in maintaining nervous system health. These associations highlight the potential for PYM as a target for therapeutic interventions in related conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845166452905,"sku":"ab263421","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263421","provider":"Iright","version":"1.0","type":"link"}