{"product_id":"abcam-ab263887","title":"Abcam, ab263887, Human Ghrelin ELISA Kit (Ghrelin-28)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Ghrelin ELISA Kit (Ghrelin-28) is a single-wash 90-min Simplestep used to quantify Human Ghrelin (Ghrelin-28) with a sensitivity of 1.048 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Heparin Plasma, Citrate plasma, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1.048 pg\/mL,\u003cbr\u003e\nRange:1.95 - 125 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Ghrelin ELISA Kit (Ghrelin-28) (ab263887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Ghrelin (Ghrelin-28) protein in cell culture media, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Ghrelin (Ghrelin-28) with 1.048 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nOur broad range of gold-standard reagents features highly specific recombinant antibodies and kits, validated across applications and species to ensure the precision and reproducibility your work requires. Drive your drug discovery efforts forward with our\u003cbr\u003e\ntailored solutions\u003cbr\u003e\ndesigned to meet the unique challenges of obesity research.\u003cbr\u003e\nGhrelin is a peptide hormone, it is a ligand of growth hormone secretagogue receptor type 1 (GHSR). It is involved in appetite stimulation and gastric acid secretion and induces adiposity. It is involved in growth regulation and induces the release of growth hormone from the pituitary. Ghrelin exists in two forms, Ghrelin-28 and one amino acid shorter Ghrelin-27. Both forms as well as Obestatin are generated by cleavage from common precursor protein, Appetite-Regulating Hormone coded by GHRL gene. Ghrelin-28 and Obestatin have non-overlapping amino acid sequences. O-octanoylation or O-decanoylation of Ghrelins is essential for Ghrelin activity.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGhrelin also known as the \"hunger hormone\" or GH secretagogue is a peptide hormone with a mass of 28 amino acids. Ghrelin is mainly expressed in the stomach particularly in the enteroendocrine cells but it can also be found in small amounts in the lungs pancreas and hypothalamus. The hormone increases food intake and promotes fat storage by signaling the brain to stimulate hunger. Its secretion occurs in response to fasting and is suppressed by food intake reflecting its role in energy balance.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGhrelin influences a variety of physiological processes beyond hunger regulation. It plays a role in modulating the release of growth hormone by acting on the growth hormone secretagogue receptor (GHS-R). Ghrelin also affects gastric motility and acid secretion due to its expression in the gastrointestinal tract. Though not part of a larger complex its interaction with GHS-R strongly influences its biological functions. Ghrelin may also impact insulin secretion and sensitivity linking it to glucose metabolism processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nGhrelin interacts with several critical biological networks. Ghrelin is involved in the neuroendocrine pathway that connects the gut to the brain and contributes significantly to energy homeostasis. Within this pathway it influences proteins like leptin which exerts opposite effects by suppressing hunger. Additionally ghrelin functions in the hedonic control of eating which relates to the rewarding aspects of food intake controlled by pathways involving dopamine.\u003cbr\u003e\nGhrelin holds relevance to obesity and cachexia a form of extreme weight loss often seen in chronic illness. Ghrelin promotes adiposity by stimulating appetite which can contribute to obesity. Conversely in cachexia the decrease in ghrelin activity is thought to exacerbate weight loss. Its role in energy balance associates ghrelin with the protein leptin which counterbalances its effects and connections to metabolic disorders. Understanding ghrelin’s functions and pathways provides insight into therapeutic strategies for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843574911145,"sku":"ab263887","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263887","provider":"Iright","version":"1.0","type":"link"}