{"product_id":"abcam-ab263920","title":"Abcam, ab263920, Human DHCR24 knockout A-431 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nDHCR24 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR\/Cas9 X = 2 bp deletion Frameshift: 99.61%.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A-431,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Skin,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by CRISPR\/Cas9 X = 2 bp deletion Frameshift: 99.61%,\u003cbr\u003e\nDisease:Epidermoid Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-DHCR24, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSeladin 1 also known as DHCR24 (3β-Hydroxysteroid-Δ24 reductase) is an enzyme with a molecular mass of approximately 54 kDa. It plays a role in cholesterol biosynthesis by catalyzing the reduction of a double bond at the C-24 position in sterol precursors. Seladin 1 is expressed in various tissues with high expression in the brain liver and adrenal gland. The enzyme contributes to the maintenance of cellular cholesterol homeostasis and provides neuroprotective functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSeladin 1 plays an important role in cellular processes particularly in response to oxidative stress. The protein is not part of a larger complex but functions independently to reduce highly reactive oxygen species levels in cells. It helps in maintaining cellular integrity and contributes to the survival of neuronal cells under stress conditions. Additionally it provides resistance to oxidative stress-induced apoptosis by modulating intracellular antioxidative defense mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeladin 1 plays an integral role in the cholesterol biosynthesis pathway and is closely associated with the mevalonate pathway. Its activity directly influences the availability of cholesterol and other sterols impacting membrane fluidity and cellular functions dependent on sterol concentrations. The enzyme interacts with related proteins such as HMG-CoA reductase an important regulatory enzyme within the cholesterol synthesis pathway. These interactions ensure that cellular cholesterol levels are well-regulated to meet physiological demands.\u003cbr\u003e\nSeladin 1 has been implicated in Alzheimer's disease and certain cancers. In Alzheimer's disease a reduction in Seladin 1 expression correlates with increased susceptibility to amyloid-beta toxicity while its overexpression shows a protective effect. This relationship ties Seladin 1 to amyloid precursor protein processing. In some cancers altered Seladin 1 expression affects tumor growth and progression where it may interact with proteins involved in cell survival pathways. This dual role in both neurodegenerative and cancerous conditions highlights the importance of Seladin 1 in human health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845115039913,"sku":"ab263920","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab263920","provider":"Iright","version":"1.0","type":"link"}