{"product_id":"abcam-ab264524","title":"Abcam, ab264524, Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nMouse Monoclonal Endothelial Cell antibody. Carrier free. Suitable for IHC-P and reacts with Rat samples. Cited in 5 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:RECA-1,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Rat,\u003cbr\u003e\nApplications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab264524 is the carrier-free version of\u003cbr\u003e\nab9774\u003cbr\u003e\nRECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-Up to 12 months, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEndothelial cells sometimes referred to as endothelium form the thin layer of approximately 1-2 µm thickness that lines the interior surface of blood vessels and lymphatic vessels. They possess a considerable array of roles mainly being a barrier between the vessel lumen and surrounding tissue and they regulate the passage of materials and the transit of white blood cells into and out of the bloodstream. Endothelial cells are widespread found in veins arteries capillaries and the heart. These cells express a variety of markers used to identify them and antibodies targeting endothelial markers are valuable tools in research and diagnostics.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEndothelial cells maintain vascular tone and blood fluidity and they mediate angiogenesis which is the development of new blood vessels from pre-existing ones. They achieve these functions through the expression of adhesion molecules and growth factors. Endothelial cells contribute to the formation of a selective permeability barrier and respond to mechanical and chemical signals. They do not function alone; they are part of a larger vascular complex that includes smooth muscle cells and pericytes.\u003cbr\u003e\nPathways\u003cbr\u003e\nEndothelial cells play key roles in the nitric oxide (NO) signaling and angiogenesis pathways. They produce nitric oxide a critical molecule for vasodilation and blood pressure regulation through the action of enzymes like endothelial nitric oxide synthase (eNOS). In the angiogenesis pathway endothelial cells interact with proteins like vascular endothelial growth factor (VEGF) which signals the proliferation and migration processes necessary for new blood vessel formation.\u003cbr\u003e\nEndothelial dysfunction links to cardiovascular conditions like atherosclerosis and hypertension. Damage or alteration in endothelial cell function can lead to abnormal vascular behavior. Oxidative stress and inflammation often disrupt endothelial activity involving proteins such as E-selectin and intercellular adhesion molecule-1 (ICAM-1) contributing to disease progression. Understanding these associations highlights the importance of endothelial cell studies in addressing cardiovascular disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848571637929,"sku":"ab264524","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab264524","provider":"Iright","version":"1.0","type":"link"}