{"product_id":"abcam-ab264604","title":"Abcam, ab264604, Human CEA ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman CEA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CEA with a sensitivity of 24.68 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 24.68 pg\/mL,\u003cbr\u003e\nRange:78.13 - 5000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman CEA ELISA Kit ab264604 is a rapid single-wash 90-min Sandwich ELISA to measure Human CEA in cell culture extracts, cell culture media, cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 24.68 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman CEA SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman CEA ELISA Kit ab264604 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-CEACAM5 antibody [EPR22676-37] - BSA and Azide free (Capture)\u003cbr\u003e\nab270312\u003cbr\u003e\n- Anti-CEACAM5 antibody [EPR22676-256] - BSA and Azide free (Detector)\u003cbr\u003e\nab270312\u003cbr\u003e\nCEA is encoded by the CECAM5 gene which produces a cell adhesion molecule consisting of an Ig variable region-like amino-terminal domain followed by up to six Ig constant region-like domains anchored to the cell membrane by either a glycosylphosphatidylinositol (GPI) moiety or a proteinaceous transmembrane and cytoplasmic domain. CEA is normally expressed on epithelial cells and is found abundantly on the apical surface of the gastrointestinal epithelium, as well as the nasopharynx, lung, urogenital tract, and sweat glands. Under physiologic conditions, only low amounts of soluble CEA can be detected in serum. However, in many different cancers, CEA is highly up regulated.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCEACAM5 also known as Carcinoembryonic Antigen (CEA) or CD66e is a glycoprotein found mainly on the surface of epithelial cells. This protein has a mass of approximately 180 kDa. It gets expressed in a variety of tissues with high expression in the gastrointestinal tract including the colon and rectum. CEACAM5 is a member of the immunoglobulin superfamily and plays a role in cell adhesion influencing how cells stick to each other.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCEACAM5 contributes to cellular processes involving cell adhesion and immune response modulation. This protein operates as part of a larger group of cell adhesion molecules and does not function as part of a complex. CEACAM5 can affect the interactions between cells and the extracellular matrix which has implications for tissue structuring and integrity. It helps the immune system recognize and respond to infectious agents or cellular anomalies.\u003cbr\u003e\nPathways\u003cbr\u003e\nCEACAM5 involvement extends to immune response regulation and cell adhesion pathways. It interacts functionally with proteins like E-cadherin in these pathways which contribute to maintaining epithelial integrity. These pathways are critical for normal tissue maintenance and immune system function especially in mucosal surfaces where pathogen entry occurs. Understanding CEACAM5 interactions provides insight into cellular communication and adhesion mechanisms.\u003cbr\u003e\nCEACAM5 is associated with colorectal cancer and lung adenocarcinoma. Its overexpression and release into the bloodstream make it a popular biomarker for these diseases. CEACAM5 is used in clinical applications such as CEA ELISA assays for cancer detection and monitoring. In colorectal cancer CEACAM5 interacts with proteins like CEACAM6 to promote tumor invasiveness and metastasis. Anti-CEACAM5 therapeutics including antibody-drug conjugates (anti-CEACAM5 ADC) are being developed to target and treat associated malignancies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843559510185,"sku":"ab264604","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab264604","provider":"Iright","version":"1.0","type":"link"}