{"product_id":"abcam-ab264792","title":"Abcam, ab264792, Human TRIB3 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nTRIB3 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 2,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TRIB3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTRIB3 also known as TRB3 or NIPK is a pseudokinase with a molecular mass of approximately 42 kDa. This protein does not possess catalytic activity. It functions largely through interactions with other proteins. TRIB3 is expressed in various tissues with high levels in the liver kidney and brain. The regulatory functions of TRIB3 involve modulating cellular processes such as stress response and apoptosis by interacting with signaling proteins.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe modulation of metabolic pathways and stress responses involves TRIB3 acting in several mechanisms. While TRIB3 itself is not part of a complex it exerts its effects by binding to and inhibiting the activity of other proteins notably AKT1 and several transcription factors. This modulation influences glucose metabolism and cellular response to unfolded protein stress indicating TRIB3's role as a significant regulator of cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe influence of TRIB3 on insulin signaling and the unfolded protein response is significant. Within the insulin signaling pathway TRIB3 interacts with AKT1 a central protein that regulates glucose uptake and cell survival. This interaction inhibits AKT1's activity impacting glucose metabolism. In the unfolded protein response pathway TRIB3 impacts stress response regulation through interactions that modulate transcriptional activity further highlighting its regulatory roles.\u003cbr\u003e\nAlterations in TRIB3 expression and activity relate to conditions such as type 2 diabetes and cancer. In type 2 diabetes increased TRIB3 levels impair insulin signaling via AKT1 inhibition contributing to insulin resistance. In cancer the dysregulation of TRIB3 can promote survival pathways circumventing apoptosis and aiding in tumor progression. The involvement of TRIB3 in these diseases highlights its potential impact in therapeutic targeting.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845082009769,"sku":"ab264792","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab264792","provider":"Iright","version":"1.0","type":"link"}