{"product_id":"abcam-ab264878","title":"Abcam, ab264878, Human MPLKIP (TTDN1) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nMPLKIP KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MPLKIP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTTDN1 also known as C20orf174 is a protein involved in cellular processes that facilitate DNA repair. It weighs approximately 47 kilodaltons and is expressed in various tissues with higher levels observed in testis and ovary. The protein plays an essential role in maintaining genomic integrity by interacting with specific components of the DNA repair machinery.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTTDN1 contributes to the repair of double-strand DNA breaks. It forms part of the larger DNA repair complex which recruits and coordinates other proteins needed for effective DNA repair. This function is especially important in cells exposed to frequent DNA damage supporting cellular stability and proper functioning by ensuring accurate DNA replication and transmission.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe functions of TTDN1 intersect with the homologous recombination and non-homologous end joining pathways. It interacts with proteins such as BRCA1 and RAD51 which are key in DNA damage response and repair. These pathways enable cells to prevent mutations therefore safeguarding the genome's integrity during cell division and proliferation.\u003cbr\u003e\nTTDN1 is associated with trichothiodystrophy a condition characterized by defects in DNA repair mechanisms. This association suggests TTDN1's involvement in clinical presentations linked to DNA repair deficiencies. Additionally anomalies in proteins like ERCC2 which also participates in DNA repair may exacerbate the effects when TTDN1 function is impaired leading to more severe disease outcomes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845158621353,"sku":"ab264878","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab264878","provider":"Iright","version":"1.0","type":"link"}