{"product_id":"abcam-ab264924","title":"Abcam, ab264924, Human SLC43A3 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSLC43A3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 3,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SLC43A3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe SLC43A3 protein also known as ENBT3 functions as a transporter for nucleobases like adenine and hypoxanthine. It belongs to the SLC43 family specifically facilitating the movement of nucleobases across the cell membrane. This protein has an approximate molecular mass of 51 kDa and is expressed in various tissues including the liver kidney and placenta. SLC43A3 plays an important role in regulating nucleotide levels within cells and maintaining a balance necessary for many cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein ensures the uptake of essential nucleobases contributing to nucleotide biosynthesis and salvage pathways. SLC43A3 does not function as part of a larger protein complex. Its role in transporting nucleobases is significant for cellular RNA and DNA synthesis indicating its involvement in cellular proliferation and repair. This highlights its importance in cellular growth and energy metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nSLC43A3 actively contributes to the purine salvage pathway critical for recycling nucleobases back into nucleotides. The purine salvage pathway is integral for conserving energy in cells as it allows the reuse of preformed bases. SLC43A3 is closely related to proteins like HPRT1 and APRT which also participate in this pathway. These interactions enable the efficient functioning of nucleotide metabolism and synthesis within various cell types.\u003cbr\u003e\nAlterations in SLC43A3 expression or function may contribute to conditions such as cancer and certain metabolic disorders. Cancer in particular often involves enhanced nucleotide synthesis and salvage pathways to support rapid cell division. SLC43A3 by adjusting nucleotide availability can influence the progression of such diseases. Its interaction with other nucleotide-related proteins can also complicate metabolic diseases. Recognizing these links is helpful for understanding the molecular basis of these diseases and potentially targeting SLC43A3 for therapeutic interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845212426409,"sku":"ab264924","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab264924","provider":"Iright","version":"1.0","type":"link"}