{"product_id":"abcam-ab265050","title":"Abcam, ab265050, Human PRKD3 (PKC nu\/PRKD3) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nPRKD3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PRKD3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nProtein kinase C nu also known as PRKD3 is an important serine\/threonine kinase located in the protein kinase D family. This protein has a molecular mass of approximately 102 kDa. PRKD3 is mainly expressed in human tissues like the brain heart and lungs. It plays a role in cellular processes by phosphorylating various substrates. The protein can localize to different cellular compartments allowing it to conduct multiple signaling roles effectively.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPRKD3 influences cellular responses to environmental stress and regulates gene expression. It can promote cell proliferation and survival by being part of a complex signaling network. This protein modulates transcription factors and cellular mechanisms to maintain homeostasis under changing conditions. PRKD3 has interactions with other kinases within its family helping to fine-tune signal transduction processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nPKC nu\/PRKD3 integrates into the MAPK signaling cascades essential for proliferative and stress-responses. It also interacts within the NF-kB pathway which regulates immune responses and inflammation. These pathways involve various other proteins like ERKs and IκB kinase positioning PRKD3 as a regulatory node in key cellular activities. This interconnection emphasizes the protein's versatile role in cellular adaptation and signaling.\u003cbr\u003e\nPRKD3 shows associations with cancer and cardiovascular diseases. The protein's influence over cell growth and apoptosis links it to oncogenic processes making it relevant in cancer research. Additionally its role in heart tissue suggests connections with cardiovascular pathologies. PRKD3 associates with proteins like Bcl-2 in cancer and influences pathways related to heart functions highlighting its relevance in these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845159538857,"sku":"ab265050","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265050","provider":"Iright","version":"1.0","type":"link"}