{"product_id":"abcam-ab265054","title":"Abcam, ab265054, Human SIRT6 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSIRT6 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon 1 and 4 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon 1 and 4 bp deletion in exon 1,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SIRT6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe SIRT6 protein also known as Sirtuin 6 plays an important role in cellular regulation. It is a member of the sirtuin family of proteins which are NAD+-dependent deacetylases. SIRT6 weighs approximately 39 kDa and can be found in the nucleus of many cell types. It acts mainly in regulating cellular homeostasis by removing acetyl groups from histone proteins which affects gene expression. Its expression levels vary across different tissues and it is known to be active in metabolically active organs such as the liver and brain.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSIRT6 influences DNA repair metabolism and inflammation. It participates in maintaining genomic stability by promoting base excision repair a critical DNA repair process. Moreover SIRT6 contributes to glucose homeostasis by influencing gluconeogenesis and glycolysis. This protein is not known to be part of any larger protein complexes but it interacts individually with other proteins to exert its biological effects.\u003cbr\u003e\nPathways\u003cbr\u003e\nSIRT6 plays a significant role in two key biological pathways: DNA damage response and metabolism regulation. In the DNA damage response pathway SIRT6 works with other proteins like PARP1 to facilitate DNA repair under stress conditions. In the regulation of metabolism SIRT6 interacts with transcription factors like HIF1α which influences the expression of genes involved in glycolytic metabolism and glucose homeostasis.\u003cbr\u003e\nSIRT6 has associations with cancer and aging-related diseases. SIRT6 has a protective effect against oncogenesis by maintaining genomic stability and regulating metabolic pathways that cancer cells exploit. Additionally its role in aging is connected to its ability to prevent age-related genomic instability and metabolic decline. In cancer SIRT6 interacts with proteins such as c-Myc and p53 influencing cell growth and apoptosis pathways. This highlights its potential as a therapeutic target for both cancer and age-related conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845067526313,"sku":"ab265054","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265054","provider":"Iright","version":"1.0","type":"link"}