{"product_id":"abcam-ab265082","title":"Abcam, ab265082, Human STK3 (MST-2) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSTK3 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-STK3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSTK3 also known as MST-2 is a serine\/threonine protein kinase with a molecular mass of around 60 kDa. It plays an integral role in cell signaling by phosphorylating and activating downstream targets. MST-2 localizes mainly in the cytoplasm and is expressed broadly across various tissues with notable activity in liver kidney and heart tissues. This kinase contributes to the regulation of cell shape and apoptosis reinforcing its importance in cellular homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSTK3\/MST-2 is a core component of the Hippo signaling pathway which controls organ size by regulating cell proliferation and apoptosis. MST-2 often interacts with other proteins and participates in functional complexes notably with MOB1 and LATS1\/2. These interactions are essential for transmitting growth inhibitory signals. Through its kinase activity MST-2 phosphorylates and activates these molecules initiating a cascade that ultimately influences gene expression impacting cell cycle and cell death processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nMST-2 plays a pivotal role not only in the Hippo pathway but also in the MAPK signaling network. In the Hippo pathway MST-2 phosphorylates components like LATS1\/2 subsequently affecting the translocation and activity of YAP\/TAZ transcription factors which control cell proliferation and survival. In the MAPK pathway MST-2 can interact with other kinases such as ASK1 to influence stress response signaling. These interactions demonstrate how MST-2 integrates signals between pathways to mediate critical decisions in cell life and death.\u003cbr\u003e\nMST-2 has implications in cancer and cardiac diseases. Dysregulation of the Hippo pathway involving MST-2 is frequently observed in various cancers where impaired MST-2 function contributes to unchecked cell growth and tumor progression. Moreover MST-2 connects with other proteins like YAP often overactive in cancers emphasizing its role in controlling cell division. In cardiac context alterations in MST-2 activity relate to cardiac hypertrophy as proper functioning of the kinase is necessary to maintain cardiac tissue architecture and response to stress.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845110714537,"sku":"ab265082","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265082","provider":"Iright","version":"1.0","type":"link"}