{"product_id":"abcam-ab265257","title":"Abcam, ab265257, Human DHRS13 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nDHRS13 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-DHRS13, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDHRS13 also known as Dehydrogenase\/Reductase Member 13 is an enzyme involved in catalyzing oxidoreductive reactions specifically acting on the CH-OH group of donors with NAD\/NADP as acceptors. The protein has an approximate mass of 35 kDa. DHRS13 shows expression in various tissues including liver kidney and adipose tissue where it likely participates in metabolic processes. As part of the short-chain dehydrogenase\/reductase (SDR) family it contributes significantly to the diverse roles related to alcohol metabolism and steroid biotransformation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe activities of DHRS13 involve critical processes related to metabolic regulation and energy homeostasis. The enzyme plays a role in lipid biosynthesis regulating fatty acid and steroid activities. Although no evidence suggests DHRS13 forms part of a larger protein complex it exerts influence on numerous cellular activities through its enzymatic actions. Despite not forming a complex it interacts indirectly with substrates and co-factors necessary for proper metabolic functioning.\u003cbr\u003e\nPathways\u003cbr\u003e\nDHRS13 integrates into cellular metabolic pathways impacting several biochemical processes. In the lipid metabolism pathway it interacts with proteins such as DHRS7 and DHRS12 contributing to the regulation of fatty acid oxidation and lipid homeostasis. Another important pathway is the NADP-dependent oxidative pathway where DHRS13's enzymatic capacity assists in balancing cellular oxidation-reduction states providing stability for metabolic controls within cells.\u003cbr\u003e\nAlterations in DHRS13 expression or activity potentially link to metabolic disorders and cancer. Researchers connect its dysregulation with cases of non-alcoholic fatty liver disease (NAFLD) where the disruption of normal lipid metabolism plays a central role. Additionally its relation to certain cancers suggests that DHRS13 might affect cellular proliferation and survival. Within these contexts DHRS13 interacts with similar enzymes including SDR family members like DHRS2 which collectively may influence cancer progression dynamics.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845199188137,"sku":"ab265257","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265257","provider":"Iright","version":"1.0","type":"link"}