{"product_id":"abcam-ab265325","title":"Abcam, ab265325, Human ARL8B knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nARL8B KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ARL8B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nARL8B also known as ADP-ribosylation factor-like protein 8B is a small GTPase with an approximate molecular mass of 22 kDa. It mainly localizes to the lysosomal membrane. This protein plays an important role in the regulation of intracellular trafficking and lysosome positioning. ARL8B is expressed across many tissues suggesting its involvement in broad cellular functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nARL8B helps manage the proper function of lysosomes by facilitating their movement and fusion with other cellular compartments. It forms a complex with other proteins such as PLEKHM2 and HOPS which are necessary for tethering events during lysosomal trafficking. This protein contributes to cellular processes such as autophagy and antigen presentation influencing the cell's ability to maintain homeostasis and effectively respond to external stimuli.\u003cbr\u003e\nPathways\u003cbr\u003e\nARL8B participates in the autophagy pathway and plays a part in the lysosome-related processes. It works closely with proteins like the kinesin motor proteins which drive the movement of lysosomes along microtubules. Through these interactions ARL8B coordinates with other autophagy-related proteins to ensure efficient degradation of cellular debris and recycling of components which are important for cellular health and function.\u003cbr\u003e\nARL8B has links to neurodegenerative diseases and immune system dysfunctions. Dysregulation of lysosomal positioning and movement caused by abnormal ARL8B function can lead to conditions such as Charcot-Marie-Tooth disease and impaired immune responses. Furthermore ARL8B interacts with proteins like Rab7a which can have implications in disease pathways emphasizing its significant role in maintaining cellular balance.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845194895529,"sku":"ab265325","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265325","provider":"Iright","version":"1.0","type":"link"}