{"product_id":"abcam-ab265481","title":"Abcam, ab265481, Human AGO1 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nAGO1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-AGO1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nArgonaute 1 (Ago1) also known as \"agoone\" is a protein that plays a central role in RNA silencing processes. It weighs around 97 kDa and is a component of the RNA-induced silencing complex (RISC). Ago1 is expressed in various tissues where it binds to small RNAs such as microRNAs (miRNAs) and small interfering RNAs (siRNAs) to guide these molecules in targeting complementary RNA sequences for gene silencing. Ago1's ability to slice or inhibit translation of target RNAs makes it important in gene expression regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAgo1 is essential in the regulation of gene expression at the post-transcriptional level. As part of the RISC complex it binds to small regulatory RNAs facilitating their role in either cleaving target RNAs or repressing their translation. This function affects numerous cellular processes including cell differentiation proliferation and development. Ago1 also interacts with other Argonaute family members like Ago2 which is directly involved in RNA cleavage. Together these proteins ensure precise regulation of gene expression.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe functional activities of Ago1 are significant in the RNA interference (RNAi) and microRNA (miRNA) pathways. In the RNAi pathway Ago1 participates by associating with siRNAs to silence target genes. In the miRNA pathway it works with miRNAs to repress translation or degrade mRNA transcripts. Ago1 collaborates with proteins like Dicer which assists in processing miRNAs and siRNAs highlighting its integrated role within these regulatory systems.\u003cbr\u003e\nDisruptions in Ago1 activity have implications in cancers and neurodegenerative diseases. In cancer altered expression of Ago1 can lead to improper regulation of gene silencing affecting tumor suppressor genes and oncogenes. In neurodegenerative diseases the dysregulation of miRNA pathways mediated by Ago1 links it to conditions like Alzheimer's disease. Other proteins like Ago2 also play roles in these diseases often working in concert with Ago1 to maintain cellular homeostasis and prevent disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845124149417,"sku":"ab265481","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265481","provider":"Iright","version":"1.0","type":"link"}