{"product_id":"abcam-ab265508","title":"Abcam, ab265508, Human MAPK7 (ERK5) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nMAPK7 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 4,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MAPK7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nERK5 also known as big MAP kinase 1 (BMK1) is an enzyme that functions as a protein-serine\/threonine kinase within the MAP kinase family. It has a molecular weight of approximately 120 kDa. ERK5 is expressed in various tissues including the brain heart and lungs with notable expression in endothelial cells. The protein plays a mechanical role in signal transduction processes which it achieves by transmitting signals from extracellular sources to the cellular nucleus facilitating the regulation of gene expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nERK5 influences cellular functions through its role in promoting cell proliferation and differentiation. It serves as part of a signaling complex that includes the MAP kinase kinase 5 (MEK5). This interaction allows ERK5 to phosphorylate and subsequently activate target substrates such as transcription factors promoting cellular responses necessary for development and survival. ERK5 also plays a role in the response to oxidative stress which contributes to its impact on survival pathways and cellular stress responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nERK5 acts within the MAPK signaling cascade and is linked to the ERK\/MAPK pathway and the phosphoinositide 3-kinase (PI3K) pathway. Within these pathways ERK5 interacts with other MAP kinases and regulatory proteins such as Ras and Raf contributing to a wide range of cellular processes including growth migration and survival. Through these interactions ERK5 influences cellular dynamics and mediates responses to growth factors and stress stimuli.\u003cbr\u003e\nAbnormal activity of ERK5 has been implicated in various conditions including cardiovascular disease and cancer. Elevated ERK5 signaling can promote tumor progression and metastasis in certain cancers by enhancing tumor cell proliferation and survival. In cardiovascular disorders ERK5 interacts with proteins like calcium\/calmodulin-dependent protein kinase II (CaMKII) influencing pathological cardiac remodeling and hypertrophy. These associations highlight ERK5's impact on disease mechanisms and highlight its potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845109633193,"sku":"ab265508","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265508","provider":"Iright","version":"1.0","type":"link"}