{"product_id":"abcam-ab265703","title":"Abcam, ab265703, Human TP53I3 (PIG3) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nTP53I3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 3,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TP53I3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPIG3 also known as PIG00 or P@IG3 str0h is a protein linked to cellular stress response with a molecular mass of approximately 55 kDa. It shows expression mainly in tissues that undergo oxidative stress or DNA damage acting as an important player in apoptosis. PIG3 is mainly known as p53-induced gene 3 aligning with its dependency on p53 for activation. High expression levels become evident in tissues encountering high metabolic or environmental stress.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPIG3 mediates cell apoptosis in response to DNA damage and oxidative stress. It forms part of a complex with p53 contributing to the regulation of apoptotic pathways. As an oxidoreductase PIG3 initiates pro-apoptotic changes within the cell. Its enzymatic activity involves producing reactive oxygen species (ROS) further amplifying the cell's apoptotic initiations. Its role connects to cellular response mechanisms in stress conditions dealing with damaged or unhealthy cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nPIG3 operates within the important p53 apoptotic pathway and the DNA damage signaling pathway. It significantly interacts with p53 as PIG3 expression depends on p53 transcription factor activation. Also PIG3 connects with caspase pathways by influencing the downstream apoptosis cascade required for cell death. It acts as a link bridging initial DNA damage response and the execution of apoptosis providing a control node within these pathways.\u003cbr\u003e\nResearch links PIG3 to cancer and neurodegenerative diseases. In cancer its expression may alter affecting cell apoptosis and proliferation balance and interacting closely with proteins like p53 and BAX. In neurodegenerative disorders abnormal PIG3 activity couples with oxidative stress that leads to neuronal degeneration. Its interplay with p53 remains significant as p53's regulation of apoptotic pathways impacts numerous conditions where PIG3's role becomes equally affected.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845210886313,"sku":"ab265703","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265703","provider":"Iright","version":"1.0","type":"link"}