{"product_id":"abcam-ab265717","title":"Abcam, ab265717, Human ATP5E knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nATP5F1E KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ATP5F1E, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nATP5E also known as ATP synthase epsilon subunit is a component of the ATP synthase enzyme complex specifically the F1 portion. This protein has an approximate molecular mass of 5.6 kDa. ATP5E is mainly present in the inner mitochondrial membrane where it plays a role in ATP production. The synthesis of ATP is a process important for cellular energy and ATP5E contributes by stabilizing the structure of the ATP synthase complex.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nATP5E functions as an integral part of the ATP synthase complex which comprises multiple subunits. This complex is essential for oxidative phosphorylation a primary method through which cells produce ATP. ATP5E's role is structural helping to keep the F1 catalytic core properly aligned during the conversion of ADP and inorganic phosphate into ATP. Through this biological activity ATP5E supports energy metabolism necessary for the survival of cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nATP5E participates in the oxidative phosphorylation pathway a critical process for cellular respiration. This pathway involves electron transport and proton pumping processes that generate the proton gradient ATP synthase exploits to synthesize ATP. Other proteins like ATP5C1 the gamma subunit are closely related to ATP5E in this pathway each contributing to the efficient production of ATP from ADP and inorganic phosphate.\u003cbr\u003e\nATP5E has connections with mitochondrial disorders such as Leigh syndrome. This disease results from mutations affecting mitochondrial function leading to severe energy deficits in cells. Mutations in ATP5E can disrupt the ATP synthase complex's function contributing to the pathology of these disorders. ATP5A1 another subunit of the ATP synthase complex also links with these diseases underlining the importance of all these components in maintaining mitochondrial integrity and function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845207183529,"sku":"ab265717","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265717","provider":"Iright","version":"1.0","type":"link"}