{"product_id":"abcam-ab265768","title":"Abcam, ab265768, Human USP54 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nUSP54 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 107 bp insertion in exon 5. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 107 bp insertion in exon 5,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-USP54, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nUSP54 also known as Ubiquitin-Specific Protease 54 is a deubiquitinating enzyme. It is part of the large family of proteases that removes ubiquitin from proteins marked for degradation therefore regulating their stability. The molecular weight of USP54 is approximately 192 kDa. Expression of USP54 occurs in a range of tissues with higher levels seen in some brain areas and liver. It has widespread but varied activity depending on cellular needs and conditions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nUSP54 plays a role in modulating protein turnover and recycling through its deubiquitinating activity. This regulation helps cells maintain balance in protein levels and activity. Unlike some other deubiquitinating enzymes USP54 is not known to be a component of a larger protein complex. However it shares functional similarities with other enzymes in the ubiquitin-proteasome system which orchestrates the targeted degradation of proteins to control cellular processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nUSP54 engages in the ubiquitin-proteasome pathway a critical pathway for protein degradation. This pathway ensures timely removal of misfolded or damaged proteins. USP54 may interact with proteins involved in this pathway to fine-tune the degradation process. Within the context of cellular regulatory networks USP54 may also have connections with signaling pathways where regulation of protein levels impacts signaling cascades. For instance proteins like UBE2D3 and UBC are ubiquitin-conjugating enzymes that might be related through shared pathway dynamics.\u003cbr\u003e\nUSP54 exhibits links to certain cancers and neurological disorders. Its role in protein regulation suggests impacts on cancer through protein stability potentially affecting cell growth and survival. Aberrant expression of USP54 may lead to altered degradation of oncogenic proteins. In neurological disorders USP54's higher expression in brain areas might suggest involvement in processes important for neural maintenance or development. The regulatory actions of USP54 might relate to proteins like p53 a known tumor suppressor protein by influencing its degradation and stability in cancer pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845199581353,"sku":"ab265768","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265768","provider":"Iright","version":"1.0","type":"link"}