{"product_id":"abcam-ab265802","title":"Abcam, ab265802, Human PPP1R13L (iASPP) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nPPP1R13L KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 4 and 2 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 4 and 2 bp deletion in exon 4,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PPP1R13L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIASPP also known as inhibitor of apoptosis-stimulating protein of p53 is a regulatory protein with a mass of approximately 96 kDa. It plays an important role in modulating apoptosis functioning as a negative regulator of p53 a well-known tumor suppressor protein. iASPP binds directly to p53 preventing its ability to transactivate target genes associated with cell cycle arrest and apoptosis. This protein is widely expressed in various tissues suggesting its importance in cellular homeostasis and response to stress.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIASPP negatively affects p53-mediated apoptosis and cellular senescence. It is part of a complex with PP1 acting as a regulatory subunit that influences several cellular processes by modulating dephosphorylation activities. iASPP's interaction with PP1 further impacts gene expression by affecting the phosphorylation status of various transcription factors. This regulatory mechanism suggests iASPP plays a role in controlling cell growth and death essential processes for maintaining normal cellular function.\u003cbr\u003e\nPathways\u003cbr\u003e\nIASPP modulates the p53 pathway an essential mechanism for regulating cell cycle and apoptosis. Within this pathway it associates with proteins like ASPP1 ASPP2 and other family members which also influence p53 activity. iASPP has a role in the NF-kB pathway where it influences inflammation and immune responses. Through these pathways iASPP coordinates signals that determine cellular fate connecting it to fundamental processes in cell biology.\u003cbr\u003e\nIASPP alterations associate with cancer development and progression. Its overexpression often correlates with enhanced tumor growth due to impaired p53 activity. Studies link iASPP to diseases like acute myeloid leukemia (AML) and prostate cancer. Through its interaction with p53 and proteins such as ASPP1 and ASPP2 iASPP provides a potential therapeutic target where modulating its activity might restore proper cellular responses to stress counteracting tumor development.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845156851881,"sku":"ab265802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265802","provider":"Iright","version":"1.0","type":"link"}