{"product_id":"abcam-ab265912","title":"Abcam, ab265912, Human HAGH (GLO2) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nHAGH KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon 3 and 4 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon 3 and 4 bp deletion in exon 3,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-HAGH, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlyoxalase 2 also known as GLO2 is an important enzyme in the glyoxalase system. The protein's molecular mass is approximately 29 kDa. It catalyzes the conversion of S-D-lactoylglutathione to glutathione and D-lactic acid allowing the detoxification of methylglyoxal a byproduct of glycolysis. GLO2 is expressed in many tissues with higher levels in liver and kidney reflecting its importance in detoxifying processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGlyoxalase 2 has a significant role in cellular detoxification and maintenance of redox balance. As part of the glyoxalase system it closely works with another enzyme glyoxalase 1 to detoxify methylglyoxal. Through this interaction GLO2 helps prevent harmful effects caused by excessive levels of advanced glycation end-products (AGEs) protecting cells from potential damage.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlyoxalase 2 is involved in the methylglyoxal degradation pathway within the cellular glucose metabolism process. Specifically it partners with glyoxalase 1 in this detoxification route. Additionally GLO2 contributes to glutathione metabolism where it plays a critical part alongside glutathione peroxidases which further regulate oxidative stress.\u003cbr\u003e\nGLO2 is associated with diabetes and neurodegenerative diseases. Altered levels of glyoxalase 2 impact methylglyoxal levels and have implications in diabetic complications through protein glycation. Furthermore studies indicate its potential link with Alzheimer's disease where oxidative stress and AGEs accumulation play central roles. GLO2's connection with glyoxalase 1 remains significant as both participate in these pathological states.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845146824873,"sku":"ab265912","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab265912","provider":"Iright","version":"1.0","type":"link"}