{"product_id":"abcam-ab266211","title":"Abcam, ab266211, Human RFX5 knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nRFX5 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 3\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RFX5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRFX5 also known as Regulatory Factor X 5 is a transcription factor characterized by a mass of approximately 74 kDa. It plays an essential role in the modulation and transcriptional regulation of genes. RFX5 is predominantly expressed in the nucleus of human cells where it binds to DNA and regulates gene expression. This protein is vital to the regulation of major histocompatibility complex (MHC) class II genes affecting how cells present antigens.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRFX5 functions as part of the RFX complex an important element for the transcription of MHC class II genes. It interacts with other regulatory factor X proteins to form this complex enabling the activation of target gene transcription. RFX5 acts by binding to the X box motifs within the promoters of the MHC class II genes. Its presence is necessary to maintain the immune response capability by ensuring the expression of these genes which is a fundamental process in antigen presentation to immune cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nRFX5 integrates into the MHC class II antigen presentation pathway. It has a significant relationship with the CIITA transcriptional regulator with CIITA enhancing the activity of RFX5 during the assembly of the MHC class II transcriptional machinery. Another important pathway is the immune response pathway where RFX5 is important in coordinating the expression of genes that help the immune system recognize and respond to pathogens effectively.\u003cbr\u003e\nDeficiencies or malfunctions in RFX5 have strong connections with Bare Lymphocyte Syndrome (BLS) type II marked by reduced expression of MHC class II molecules. In this context RFX5's interactions with CIITA are critical because improper function of either can lead to compromised immune responses. Studies also suggest an association between disrupted RFX5 activity and autoimmune diseases where improper antigen presentation can lead to the immune system attacking the body's own tissues.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845195911337,"sku":"ab266211","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab266211","provider":"Iright","version":"1.0","type":"link"}