{"product_id":"abcam-ab266247","title":"Abcam, ab266247, Human PCNP knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nPCNP KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 13 bp deletion in exon 1 and 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 13 bp deletion in exon 1 and 1 bp insertion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PCNP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPCNP also called PEST-containing nuclear protein consists of around 178 amino acids and has a mass of approximately 21 kDa. PCNP localizes mainly in the nucleus of cells and shows expression in various tissues including the testis and skeletal muscle. The presence of PEST sequences suggests the target may experience rapid degradation which is typical for proteins that regulate essential cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein participates in cell cycle regulation and ubiquitination pathways suggesting its role in protein turnover within cells. PCNP often interacts with other proteins as part of larger complexes to influence proteolysis and cell cycle progression. The interaction with molecules containing ubiquitin-like domains indicates its involvement in tagging specific proteins for degradation helping maintain cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nPCNP connects strongly to the ubiquitin-proteasome pathway and the regulation of transcription. Its interactions with proteins such as RNF115 indicate its role in protein ubiquitination a fundamental process for maintaining cell integrity and function. Another relevant link is to cyclin-dependent kinases suggesting a contribution to cell cycle checkpoints and transitions essential for controlled cell division and proliferation.\u003cbr\u003e\nPCNP's role highlights its potential involvement in oncogenesis and neurodegenerative disorders. Aberrant expression or regulation can connect PCNP to cancer progression by influencing the degradation of cell cycle regulators. Additionally defects in ubiquitination processes can tie this protein to conditions such as Alzheimer's disease where misfolded proteins accumulate. Interaction with proteins like beta-amyloid precursor protein suggests a possible link to neurodegenerative pathways impacting both disease development and progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845154754729,"sku":"ab266247","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab266247","provider":"Iright","version":"1.0","type":"link"}