{"product_id":"abcam-ab266430","title":"Abcam, ab266430, Human EIF4H knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nEIF4H KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-EIF4H, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe eukaryotic translation initiation factor 4H commonly known as eIF4H is an essential protein participating in the initiation phase of protein synthesis. It holds a mass of approximately 27 kDa. eIF4H is expressed in various tissues including the brain liver and muscle. The protein promotes the unwinding of RNA secondary structures aiding ribosome attachment to the mRNA. This function aligns closely with its alternate name eukaryotic translation initiation factor 4H.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEIF4H enhances the helicase activity of eIF4A an RNA helicase vital for translation initiation. It forms part of the translation initiation complex. The complex brings together eIF4E eIF4G and eIF4A enhancing mRNA recognition and processing. eIF4H stabilizes the eIF4A-mRNA interaction reducing the likelihood of premature dissociation and permitting efficient scanning for the start codon.\u003cbr\u003e\nPathways\u003cbr\u003e\nEIF4H plays a significant role in the mTOR (mechanistic target of rapamycin) signaling pathway which regulates cell growth and proliferation. This pathway influences protein synthesis by controlling the availability and activity of initiation factors like eIF4A and eIF4E. mTOR signaling overlaps with the MAPK (mitogen-activated protein kinase) pathway with eIF4H indirectly interacting with RAF proteins which regulate cell cycle progression and differentiation.\u003cbr\u003e\nResearchers link eIF4H to neurological disorders like Autism Spectrum Disorder and certain cancers such as glioblastoma. Alterations in the expression or function of eIF4H may impact the mTOR pathway influencing tumor progression and neural development. These dysfunctional roles also involve eIF4E as both proteins contribute to abnormal translation initiation and gene expression in these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845207347369,"sku":"ab266430","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab266430","provider":"Iright","version":"1.0","type":"link"}