{"product_id":"abcam-ab266596","title":"Abcam, ab266596, Human OSTC knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nOSTC KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 7 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 7 bp deletion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-OSTC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nOSTC also known as oligosaccharyltransferase complex subunit is a component in the N-linked glycosylation pathway. It has a molecular mass of approximately 16 kDa. OSTC is located primarily in the endoplasmic reticulum and displays a ubiquitous expression indicating its presence in various cell types and tissues. This protein is an important player in the assembly and function of the oligosaccharyltransferase complex which catalyzes the transfer of oligosaccharides onto nascent peptides a process important for proper protein folding and stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe oligosaccharyltransferase complex including OSTC plays a significant role in protein modification. The complex facilitates the attachment of glycans to asparagine residues on target proteins an essential post-translational modification process. Being a part of this multi-protein complex OSTC contributes actively to maintaining cellular homeostasis by influencing protein quality control and secretion.\u003cbr\u003e\nPathways\u003cbr\u003e\nOSTC's involvement is embedded in the N-glycosylation pathway which is important for the proper folding and function of numerous glycoproteins. This pathway intersects with the protein processing pathway in the endoplasmic reticulum where OSTC collaborates with other protein components like OST48 and ribophorin ensuring efficient protein maturation. These interactions highlight OSTC's role in maintaining cellular function through its integration into key biochemical pathways.\u003cbr\u003e\nSeveral studies link OSTC to congenital disorders of glycosylation which arise from dysfunctional glycosylation processes. Abnormal OSTC expression or function may result in disrupted protein folding and stability contributing to neurodevelopmental disorders and immunodeficiencies. Furthermore OSTC's interaction with proteins involved in the glycosylation pathway can accentuate phenotypic manifestations in ER stress-related diseases highlighting the importance of this target in clinical research and therapy development.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845195354281,"sku":"ab266596","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab266596","provider":"Iright","version":"1.0","type":"link"}