{"product_id":"abcam-ab266667","title":"Abcam, ab266667, Human Arhgef1 (p115-RhoGEF) knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nARHGEF1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 3\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ARHGEF1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe p115-RhoGEF also known as ARHGEF1 is a guanine nucleotide exchange factor that primarily activates RhoA a small GTPase. It has a molecular mass of approximately 115 kDa. You find p115-RhoGEF predominantly expressed in the heart lung and brain tissues. This protein facilitates the exchange of GDP for GTP on RhoA triggering downstream signaling events essential for cellular functions like growth motility and organization of cytoskeletal structures.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nP115-RhoGEF plays an important role in regulating actin cytoskeleton dynamics and transmitting signals from G-protein-coupled receptors. The protein is part of a signaling complex that includes heterotrimeric G proteins. Through its influence on the actin cytoskeleton p115-RhoGEF helps control changes in cell shape and movement. These activities contribute significantly to various cellular processes including differentiation and migration.\u003cbr\u003e\nPathways\u003cbr\u003e\nP115-RhoGEF is an essential component in the RhoA signaling pathway. This pathway regulates cytoskeletal dynamics and has critical implications for processes like cell adhesion and migration. p115-RhoGEF collaborates with other proteins such as Gα12\/13 subunits to mediate its signaling effects. Besides the RhoA pathway p115-RhoGEF also interacts with the PI3K\/Akt pathway influencing cellular survival and apoptosis.\u003cbr\u003e\nDisruptions in p115-RhoGEF activity link to cardiovascular diseases and certain forms of cancer. Alterations in its function may lead to improper cell movement contributing to metastasis in cancer development. Additionally abnormal regulation of RhoA signaling connected to p115-RhoGEF could compromise vascular integrity implicating this protein in hypertension. During disease progression p115-RhoGEF interacts with other proteins like ROCK1 to exert these pathological effects.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845080076457,"sku":"ab266667","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab266667","provider":"Iright","version":"1.0","type":"link"}