{"product_id":"abcam-ab266679","title":"Abcam, ab266679, Human ITPA knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nITPA KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ITPA, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nITPA also known as inosine triphosphate pyrophosphatase is an important enzyme responsible for catalyzing the hydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP). This enzyme prevents the accumulation of non-canonical nucleotides in the cell. ITPA has a mass of approximately 21 kDa and is expressed ubiquitously in various tissues indicating its importance across different cell types. The enzyme is also present in HEK 293T cells as researchers often use these cells for studies due to their growth characteristics and transfection efficiency.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme plays a pivotal role in nucleotide metabolism by regulating the levels of ITP deoxyinosine triphosphate (dITP) and xanthosine triphosphate (XTP). By hydrolyzing these non-standard nucleotides ITPA maintains genomic integrity and prevents mutations during DNA replication. It is not typically considered part of a larger complex but acts independently to safeguard the nucleotide pool from potentially harmful nucleotide analogs.\u003cbr\u003e\nPathways\u003cbr\u003e\nITPA's activity is essential in the purine metabolism pathway where it helps maintain a proper balance of nucleotide synthesis and degradation. This function helps prevent the incorporation of non-standard bases into DNA and RNA which can lead to genetic instability. ITPA activity is also intertwined with the salvage pathway of purine nucleotides. This connection highlights its indirect relationship with proteins involved in nucleotide synthesis and repair such as DNA polymerases and other nucleotide-metabolizing enzymes.\u003cbr\u003e\nITPA has associations with certain pathological conditions. For example ITPA deficiency can lead to inosine triphosphate accumulation which might contribute to hemolytic anemia. Mutations in the ITPA gene can also impact drug metabolism notably in patients receiving thiopurine drugs such as those used for leukemia treatment. These mutations may lead to adverse drug reactions due to the altered nucleotide balance. The connection with these conditions emphasizes the role of ITPA in maintaining cellular homeostasis and its potential influence on pharmacogenomics.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845069263017,"sku":"ab266679","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab266679","provider":"Iright","version":"1.0","type":"link"}