{"product_id":"abcam-ab266699","title":"Abcam, ab266699, Human FLAD1 knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nFLAD1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 3\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-FLAD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFLAD1 also known as FAD synthase is an enzyme that plays a role mechanically in the synthesis of Flavin Adenine Dinucleotide (FAD) from ATP and FMN (Flavin Mononucleotide). FLAD1 has an approximate mass of 66 kDa. It is expressed in a variety of tissues with high levels in liver heart and kidneys. Its primary responsibility is to ensure the sufficient production of FAD necessary for various metabolic enzymes and biological processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFLAD1 facilitates essential cellular processes by enabling the synthesis of FAD a cofactors required for the functioning of oxidoreductase enzymes involved in redox reactions. FAD forms part of several enzyme complexes vital for energy production and cellular respiration including those complexes located within the mitochondria. FLAD1's action allows cells to maintain metabolic activities such as the Krebs cycle and the electron transport chain.\u003cbr\u003e\nPathways\u003cbr\u003e\nFlavin Adenine Dinucleotide produced by FLAD1 is fundamental for proper mitochondrial function through participation in the oxidative phosphorylation and the beta-oxidation pathways. These pathways are critical for efficient ATP production. Related proteins that also contribute to these pathways include succinate dehydrogenase and acyl-CoA dehydrogenase which depend on FAD to perform their metabolic roles.\u003cbr\u003e\nDisruptions in FLAD1 function can contribute to metabolic-related conditions. The dysfunctional production of FAD can associate with mitochondrial disorders where energy production is impaired and FLAD1 mutations are linked to conditions like multiple acyl-CoA dehydrogenase deficiency (MADD). Proteins such as ETFDH another enzyme involved in the electron transport chain have connections with FLAD1 especially in these metabolic disorders highlighting the enzyme's importance in maintaining normal cellular function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845209575593,"sku":"ab266699","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab266699","provider":"Iright","version":"1.0","type":"link"}